Product Name :
YTHDF2 Recombinant Rabbit Monoclonal Antibody [PSH06-17]

Predicted band size :
62 kDa

Observed band size :
62 kDa

Synonyms:
9430020E02Rik antibody CAHL antibody CLL associated antigen KW 14 antibody CLL-associated antigen KW-14 antibody HGRG8 antibody High glucose regulated protein 8 antibody High-glucose-regulated protein 8 antibody NY REN 2 antibody Renal carcinoma antigen NY REN 2 antibody Renal carcinoma antigen NY-REN-2 antibody 展开

Function :
Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates their stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing. Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT and ribonuclease P/MRP complexes, depending on the context. The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) share m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation. M6A-containing mRNAs containing a binding site for RIDA/HRSP12 (5′-GGUUC-3′) are preferentially degraded by endoribonucleolytic cleavage: cooperative binding of RIDA/HRSP12 and YTHDF2 to transcripts leads to recruitment of the ribonuclease P/MRP complex. Other m6A-containing mRNAs undergo deadenylation via direct interaction between YTHDF2 and CNOT1, leading to recruitment of the CCR4-NOT and subsequent deadenylation of m6A-containing mRNAs. Required maternally to regulate oocyte maturation: probably acts by binding to m6A-containing mRNAs, thereby regulating maternal transcript dosage during oocyte maturation, which is essential for the competence of oocytes to sustain early zygotic development.Chroman-7-amine web Also required during spermatogenesis: regulates spermagonial adhesion by promoting degradation of m6A-containing transcripts coding for matrix metallopeptidases.Boc-NH-PEG4-CH2CH2NH2 site Also involved in hematopoietic stem cells specification by binding to m6A-containing mRNAs, leading to promote their degradation.PMID:33449491 Also acts as a regulator of neural development by promoting m6A-dependent degradation of neural development-related mRNA targets.

Antibody Type:
Recombinant Rabbit monoclonal Antibody

Immunogen :
Synthetic peptide within Human YTHDF2 aa 519-579.

Species reactivity:
Human, Mouse, Rat, Monkey

Verify application:
WB, IHC-P, FC

Coupling :
unconjugated

Clone number:
PSH06-17

RRID:

Form :
Liquid

Concentration :
1ug/ul

Storage instructions:
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage buffer solution:
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Subtype:
IgG

Purification method :
Protein A affinity purified.

ELTSA :

Molecular weight :
Predicted band size: 62 kDa

Positive control :
HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, HCT 116 cell lysate, MDA-MB-231 cell lysate, COS-1 cell lysate, human testis tissue, mouse brain tissue, mouse testis tissue, rat brain tissue, rat testis tissue, HCT 116.